Studying endocytosis in space and time by means of temporal Boolean models

  • Authors:
  • R. Sebastian;E. Díaz;G. Ayala;M. E. Díaz;R. Zoncu;D. Toomre

  • Affiliations:
  • Departamento de Informática, Universidad de Valencia, Doctor Moliner, 50, 46100 Burjasot, Spain;Departamento de Informática, Universidad de Valencia, Doctor Moliner, 50, 46100 Burjasot, Spain;Departamento de Estadística e Investigación Operativa, Universidad de Valencia, Doctor Moliner, 50, 46100 Burjasot, Spain;Departamento de Informática, Universidad de Valencia, Doctor Moliner, 50, 46100 Burjasot, Spain;Department of Cell Biology, Yale University, 333 Cedar Street, P.O. Box 208002, New Haven, CT 06520-8002, USA;Department of Cell Biology, Yale University, 333 Cedar Street, P.O. Box 208002, New Haven, CT 06520-8002, USA

  • Venue:
  • Pattern Recognition
  • Year:
  • 2006

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Abstract

Endocytosis is a process by which cells carry traffic from the extracellular space into various intracellular compartments. Visualization of fluorescently tagged clathrin proteins (mediators of endocytosis) allows us to image endocytosis in real time. When imaging the plasma membrane, areas of fluorescence generated by different endocytic processes overlap spatially and temporally, forming random clumps. Here, a sequence of segmented clathrin spots is considered a realization of a non-isotropic 3D Boolean model. Estimates of the intensity, the mean perimeter and the density function of the durations of endocytic events are obtained.