Segmentation and border identification of cells in images of peripheral blood smear slides

  • Authors:
  • Nicola Ritter;James Cooper

  • Affiliations:
  • Murdoch University, Perth, W.A., Australia;Curtin University of Technology, Perth, W.A., Australia

  • Venue:
  • ACSC '07 Proceedings of the thirtieth Australasian conference on Computer science - Volume 62
  • Year:
  • 2007

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Abstract

We present an unsupervised blood cell segmentation algorithm for images taken from peripheral blood smear slides. Unlike prior algorithms the method is fast; fully automated; finds all objects---cells, cell groups and cell fragments---that do not intersect the image border; identifies the points interior to each object; finds an accurate one pixel wide border for each object; separates objects that just touch; and has been shown to work with a wide selection of red blood cell morphologies. The full algorithm was tested on two sets of images. In the first set of 47 images, 97.3% of the 2962 image objects were correctly segmented. The second test set---51 images from a different source---contained 5417 objects for which the success rate was 99.0%. The time taken for processing a 2272x1704 image ranged from 4.86 to 11.02 seconds on a Pentium 4, 2.4 GHz machine, depending on the number of objects in the image.