Hierarchical SOMs: Segmentation of Cell-Migration Images

  • Authors:
  • Chaoxin Zheng;Khurshid Ahmad;Aideen Long;Yuri Volkov;Anthony Davies;Dermot Kelleher

  • Affiliations:
  • Department of Computer Science, O'Reilly Institute, Trinity College Dublin, Dublin 2, Ireland;Department of Computer Science, O'Reilly Institute, Trinity College Dublin, Dublin 2, Ireland;Department of Clinical Medicine, Trinity College & Dublin Molecular Medicine Centre, St. James's Street, Dublin 8, Ireland;Department of Clinical Medicine, Trinity College & Dublin Molecular Medicine Centre, St. James's Street, Dublin 8, Ireland;Department of Clinical Medicine, Trinity College & Dublin Molecular Medicine Centre, St. James's Street, Dublin 8, Ireland;Department of Clinical Medicine, Trinity College & Dublin Molecular Medicine Centre, St. James's Street, Dublin 8, Ireland

  • Venue:
  • ISNN '07 Proceedings of the 4th international symposium on Neural Networks: Part II--Advances in Neural Networks
  • Year:
  • 2007

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Abstract

The application of hierarchical self organizing maps (HSOM) to the segmentation of cell migration images, obtained during high-content screening in molecular medicine, is described. The segmentation is critical to our larger project for developing methods for the automatic annotation of cell migration images. The HSOM appears to perform better than the conventional computer-vision methods of histogram thresholding, edge detection, and the newer techniques involving single-layer SOMs. However, the HSOM techniques have to be complemented by region-based techniques to improve the quality of the segmented images.