Segmentation through Variable-Order Surface Fitting
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Evaluation of Methods for Ridge and Valley Detection
IEEE Transactions on Pattern Analysis and Machine Intelligence
Moving object segmentation using the flux tensor for biological video microscopy
PCM'07 Proceedings of the multimedia 8th Pacific Rim conference on Advances in multimedia information processing
Cell segmentation using coupled level sets and graph-vertex coloring
MICCAI'06 Proceedings of the 9th international conference on Medical Image Computing and Computer-Assisted Intervention - Volume Part I
Automatic Segmentation of High-Throughput RNAi Fluorescent Cellular Images
IEEE Transactions on Information Technology in Biomedicine
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Segmentation and Classification of Cell Cycle Phases in Fluorescence Imaging
MICCAI '09 Proceedings of the 12th International Conference on Medical Image Computing and Computer-Assisted Intervention: Part II
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Cell adhesion and spreading within the extracellular matrix (ECM) plays an important role in cell motility, cell growth and tissue organization. Measuring cell spreading dynamics enables the investigation of cell mechanosensitivity to external mechanical stimuli, such as substrate rigidity. A common approach to measure cell spreading dynamics is to take time lapse images and quantify cell size and perimeter as a function of time. In our experiments, differences in cell characteristics between different treatments are subtle and require accurate measurements of cell parameters across a large population of cells to ensure an adequate sample size for statistical hypothesis testing. This paper presents a new approach to estimate accurate cell boundaries with complex shapes by applying a modified geodesic active contour level set method that directly utilizes the halo effect typically seen in phase contrast microscopy. Contour evolution is guided by edge profiles in a perpendicular direction to ensure convergence to the correct cell boundary. The proposed approach is tested on bovine aortic endothelial cell images under different treatments, and demonstrates accurate segmentation for a wide range of cell sizes and shapes compared to manual ground truth.