Statistical thermodynamic analysis and designof DNA-based computers
Natural Computing: an international journal
Problems of Information Transmission
Characterization of Non-crosshybridizing DNA Oligonucleotides Manufactured in vitro
Natural Computing: an international journal
An approach for using modified nucleotides in aqueous DNA computing
DNA13'07 Proceedings of the 13th international conference on DNA computing
Experimental validation of DNA sequences for DNA computing: use of a SYBR green I assay
DNA'05 Proceedings of the 11th international conference on DNA Computing
Algorithmic applications of XPCR
Natural Computing: an international journal
Natural Computing: an international journal
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DNA code sequences generated by the program SynDCode were used to construct a 5-site, 2-variable computational DNA library by parallel overlap self-assembly. The final library was amplified using the polymerase chain reaction (PCR) to obtain a fragment of the expected size. Twelve library sequences randomly selected from the library by cloning were sequenced and found to be distinct and correctly assembled library strands. A Birthday Problem-like analysis suggests that we have all 32 different molecules in our library mixture. We then developed new protocols using DNA hybridization to successfully identify single members of this library. We have also used this protocol to analyze mixtures of clones from the library. This approach shows the experimental validation of the ability to distinguish different sequences generated from the SynDCode program. We are in the process of working out protocols to separate out specific library members and to expand this library.