In silico analysis of motifs in promoters of Differentially Expressed Genes in rice (Oryza sativa L.) under anoxia

  • Authors:
  • Ashutosh Kumar;Shuchi Smita;Neeti Sahu;Vivekanand Sharma; Shankaracharya;Ambarish S. Vidyarthi;Dev Mani Pandey

  • Affiliations:
  • Department of Biotechnology, Birla Institute of Technology, Mesra, Ranchi 835 215, Jharkhand, India.;Department of Biotechnology, Birla Institute of Technology, Mesra, Ranchi 835 215, Jharkhand, India.;Department of Biotechnology, Birla Institute of Technology, Mesra, Ranchi 835 215, Jharkhand, India.;Department of Plant Biology, Marsh Life Sciences Building, University of Vermont, Burlington 05405 Vermont, USA.;Department of Biotechnology, Birla Institute of Technology, Mesra, Ranchi 835 215 Jharkhand, India.;Department of Biotechnology, Birla Institute of Technology, Mesra, Ranchi 835 215 Jharkhand, India.;Department of Biotechnology, Birla Institute of Technology, Mesra, Ranchi 835 215 Jharkhand, India

  • Venue:
  • International Journal of Bioinformatics Research and Applications
  • Year:
  • 2009

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Abstract

The aim of this study was to characterise the molecular mechanisms of transcriptional regulation of Differentially Expressed Genes (DEGs) in rice coleoptiles under anoxia by identifying motifs that are common in the promoter region of co-regulated genes. Un-changed DEGs (<2 fold and >−2), up-regulated DEGs (≥2 fold) and down-regulated DEGs (≤2 fold) were separated in three different data sets. Their gene promoters were extracted from eukaryotic promoter database. Statistically significant consensus promoter motifs were detected by in silico method. A significant variation in the number of promoter motifs, consensus promoter motif and their sequences between UR-DEGs and DR-DEGs were detected that might be responsible for their related expression.