Intelligent splitting in the chromosome domain
Pattern Recognition
IEEE Transactions on Pattern Analysis and Machine Intelligence
A curvature-based multiresolution automatic karyotyping system
Machine Vision and Applications
New features for automatic classification of human chromosomes: A feasibility study
Pattern Recognition Letters
Disentangling chromosome overlaps by combining trainable shapemodels with classification evidence
IEEE Transactions on Signal Processing
IEEE Transactions on Signal Processing
Toward a completely automatic neural-network-based human chromosomeanalysis
IEEE Transactions on Systems, Man, and Cybernetics, Part B: Cybernetics
A modular framework for the automatic classification of chromosomes in Q-band images
Computer Methods and Programs in Biomedicine
A review of thresholding strategies applied to human chromosome segmentation
Computer Methods and Programs in Biomedicine
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Karyotype analysis is a widespread procedure in cytogenetics to assess the possible presence of genetics defects. The procedure is lengthy and repetitive, so that an automatic analysis would greatly help the cytogeneticist routine work. Still, automatic segmentation and full disentangling of chromosomes are open issues. We propose an automatic procedure to obtain the separated chromosomes, which are then ready for a subsequent classification step. The segmentation is carried out by means of a space-variant thresholding scheme, which proved to be successful even in presence of hyper- or hypofluorescent regions in the image. Then, the tree of choices to resolve touching and overlapping chromosomes is recursively explored, choosing the best combination of cuts and overlaps based on geometric evidence and image information. We show the effectiveness of the proposed method on routine data acquired with different microscope-camera setup at different laboratories: from 162 images of 117 cells totaling 6683 chromosomes, 94% of the chromosomes were correctly segmented, solving 90% of the overlaps and 90% of the touchings. In order to provide the scientific community with a public dataset, the data used in this paper are available for public download.