Intelligent splitting in the chromosome domain
Pattern Recognition
IEEE Transactions on Pattern Analysis and Machine Intelligence
Pattern Recognition with Fuzzy Objective Function Algorithms
Pattern Recognition with Fuzzy Objective Function Algorithms
Bilateral Filtering for Gray and Color Images
ICCV '98 Proceedings of the Sixth International Conference on Computer Vision
A curvature-based multiresolution automatic karyotyping system
Machine Vision and Applications
Optimal multi-thresholding using a hybrid optimization approach
Pattern Recognition Letters
A multistage adaptive thresholding method
Pattern Recognition Letters
A multi-level thresholding approach using a hybrid optimal estimation algorithm
Pattern Recognition Letters
Automatic segmentation and disentangling of chromosomes in Q-band prometaphase images
IEEE Transactions on Information Technology in Biomedicine - Special section on biomedical informatics
A modular framework for the automatic classification of chromosomes in Q-band images
Computer Methods and Programs in Biomedicine
IEEE Transactions on Signal Processing
Toward a completely automatic neural-network-based human chromosomeanalysis
IEEE Transactions on Systems, Man, and Cybernetics, Part B: Cybernetics
Minimization of Region-Scalable Fitting Energy for Image Segmentation
IEEE Transactions on Image Processing
Cell tracking in microscopic video using matching and linking of bipartite graphs
Computer Methods and Programs in Biomedicine
Teeth segmentation of dental periapical radiographs based on local singularity analysis
Computer Methods and Programs in Biomedicine
A marker-based watershed method for X-ray image segmentation
Computer Methods and Programs in Biomedicine
Preparation of 2D sequences of corneal images for 3D model building
Computer Methods and Programs in Biomedicine
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Karyotype analysis is a widespread procedure in cytogenetics to assess the presence of genetic defects by the visualization of the structure of chromosomes. The procedure is lengthy and repetitive and an effective automatic analysis would greatly help the cytogeneticist routine work. Still, automatic segmentation and the full disentangling of chromosomes are open issues. The first step in every automatic procedure is the thresholding step, which detect blobs that represent either single chromosomes or clusters of chromosomes. The better the thresholding step, the easier is the subsequent disentanglement of chromosome clusters into single entities. We implemented eleven thresholding methods, i.e. the ones that appear in the literature as the best performers, and compared their performance in segmenting chromosomes and chromosome clusters in cytogenetic Q-band images. The images are affected by the presence of hyper- or hypo-fluorescent regions and by a contrast variability between the stained chromosomes and the background. A thorough analysis of the results highlights that, although every single algorithm shows peculiar strong/weak points, Adaptive Threshold and Region Based Level Set have the overall best performance. In order to provide the scientific community with a public dataset, the data and manual segmentation used in this paper are available for public download at http://bioimlab.dei.unipd.it