BIBE '01 Proceedings of the 2nd IEEE International Symposium on Bioinformatics and Bioengineering
Journal of VLSI Signal Processing Systems - Special issue on signal processing and neural networks for bioinformatics
Automatic Segmentation of Unstained Living Cells in Bright-Field Microscope Images
MDA '08 Proceedings of the 3rd international conference on Advances in Mass Data Analysis of Images and Signals in Medicine, Biotechnology, Chemistry and Food Industry
ISBI'09 Proceedings of the Sixth IEEE international conference on Symposium on Biomedical Imaging: From Nano to Macro
Invariance via group-integration: a feature framework for 3D biomedical image analysis
CGIM '08 Proceedings of the Tenth IASTED International Conference on Computer Graphics and Imaging
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Detailed knowledge of the subcellular location of all proteins and how they change under various conditions is essential for systems biology efforts to recreate the behavior of cells and organisms. Systematic study of sub-cellular patterns requires automated methods to determine the location pattern for each protein and how it relates to others. Our group has designed sets of numerical features that characterize the location patterns in high-resolution fluorescence microscope images, has shown that these can be used to distinguish patterns better than visual examination, and has used them to automatically group proteins by their patterns. In the current study, we sought to extend our approaches to images obtained from different cell types, microscopy techniques and resolutions. The results indicate that 1) transformation of subcellular location features can be performed so that similar patterns from different cell types are grouped by automated clustering; and 2) there are several basic location patterns whose recognition is insensitive to image resolution over a wide range. The results suggest strategies to be used for collecting and analyzing images from different cell types and with different resolutions.