Searching Online Journals for Fluorescence Microscope Images Depicting Protein Subcellular Location Patterns

Authors:
Robert F. Murphy;Meel Velliste;Jie Yao;Gregory Porreca
Affiliations:
-;-;-;-
Venue:
BIBE '01 Proceedings of the 2nd IEEE International Symposium on Bioinformatics and Bioengineering
Year:
2001

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Abstract

There is extensive interest in automating the collection, organization and analysis of biological data. Data in the form of images present special challenges for such efforts. Since fluorescence microscope images are a primary source of information about the location of proteins within cells, we have set as a long-term goal the building of a knowledge base system that can interpret such images in online journals. To this end, we first developed a robot that searches online journals and finds fluorescence microscope images of individual cells. We then characterized the applicability of pattern classification methods we have previously used on images obtained under controlled conditions to images from different sources and to images subjected to manipulations commonly performed during publication. The results indicate the feasibility of developing search engines to find fluorescence microscope images depicting particular subcellular patterns.