Journal of Combinatorial Theory Series B - Special issue: dedicated to Professor W. T. Tutte on the occasion of his eightieth birthday
Geodesic Active Contours and Level Sets for the Detection and Tracking of Moving Objects
IEEE Transactions on Pattern Analysis and Machine Intelligence
A Multiphase Level Set Framework for Image Segmentation Using the Mumford and Shah Model
International Journal of Computer Vision
Level Set Evolution without Re-Initialization: A New Variational Formulation
CVPR '05 Proceedings of the 2005 IEEE Computer Society Conference on Computer Vision and Pattern Recognition (CVPR'05) - Volume 1 - Volume 01
Coupled Parametric Active Contours
IEEE Transactions on Pattern Analysis and Machine Intelligence
Cell segmentation using coupled level sets and graph-vertex coloring
MICCAI'06 Proceedings of the 9th international conference on Medical Image Computing and Computer-Assisted Intervention - Volume Part I
IEEE Transactions on Image Processing
Level set analysis for leukocyte detection and tracking
IEEE Transactions on Image Processing
Segmenting and tracking fluorescent cells in dynamic 3-D microscopy with coupled active surfaces
IEEE Transactions on Image Processing
Accurate spatial neighborhood relationships for arbitrarily-shaped objects using Hamilton-Jacobi GVD
SCIA'07 Proceedings of the 15th Scandinavian conference on Image analysis
Moving object segmentation using the flux tensor for biological video microscopy
PCM'07 Proceedings of the multimedia 8th Pacific Rim conference on Advances in multimedia information processing
Fast graph partitioning active contours for image segmentation using histograms
Journal on Image and Video Processing
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Understanding behavior of migrating cells is becoming an emerging research area with many important applications. Segmentation and tracking constitute vital steps of this research. In this paper, we present an automated cell segmentation and tracking system designed to study migration of cells imaged with a phase contrast microscope. For segmentation the system uses active contour level set methods with a novel extension that efficiently prevents false-merge problem. Tracking is done by resolving frame to frame correspondences between multiple cells using a multi-distance, multi-hypothesis algorithm. Cells that move into the field-of-view, arise from cell division or disappear due to apoptosis are reliably segmented and tracked by the system. Robust tracking of cells, imaged with a phase contrast microscope is a challenging problem due to difficulties in segmenting dense clusters of cells. As cells being imaged have vague borders, close neighboring cells may appear to merge. These false-merges lead to incorrect trajectories being generated during the tracking process. Current level-set based approaches to solve the false-merge problem require a unique level set per object (the N-level set paradigm). The proposed approach uses evidence from previous frames and graph coloring principles and solves the same problem with only four level sets for any arbitrary number of similar objects, like cells.