Successive State Transitions with I/O Interface by Molecules
DNA '00 Revised Papers from the 6th International Workshop on DNA-Based Computers: DNA Computing
DNA 7 Revised Papers from the 7th International Workshop on DNA-Based Computers: DNA Computing
Genetic Programming and Evolvable Machines
Statistical thermodynamic analysis and designof DNA-based computers
Natural Computing: an international journal
DNA'06 Proceedings of the 12th international conference on DNA Computing
Isothermal reactivating Whiplash PCR for locally programmable molecular computation
Natural Computing: an international journal
Experimental validation and optimization of signal dependent operation in whiplash PCR
Natural Computing: an international journal
Design of a biomolecular device that executes process algebra
Natural Computing: an international journal
DNA'06 Proceedings of the 12th international conference on DNA Computing
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Whiplash PCR-based methods of biomolecular computation (BMC), while highly-versatile in principle, are well-known to suffer from a simple but serious form of self-poisoning known as back-hybridization. In this work, an optimally re-engineered WPCR-based architecture, Displacement Whiplash PCR (DWPCR) is proposed and experimentally validated. DWPCR’s new rule protect biostep, which is based on the primer-targeted strand-displacement of back-hybridized hairpins, renders the most recently implemented rule-block of each strand unavailable, abolishing back-hybridization after each round of extension. In addition to attaining a near-ideal efficiency, DWPCR’s ability to support isothermal operation at physiological temperatures eliminates the need for thermal cycling, and opens the door for potential biological applications. DWPCR should also be capable of supporting programmable exon shuffling, allowing XWPCR, a proposed method for programmable protein evolution, to more closely imitate natural evolving systems. DWPCR is expected to realize a highly-efficient, versatile platform for routine and efficient massively parallel BMC.