Characterization of non-crosshybridizing DNA oligonucleotides manufactured In Vitro

  • Authors:

  • Junghuei Chen;Russell Deaton;Max H. Garzon;Jin Woo Kim;David Wood;Hong Bi;Dylan Carpenter;Yu-Zhen Wang

  • Affiliations:

  • Chemistry and Biochemistry, University of Delaware, Newark, DE;Computer Science and Engineering, University of Arkansas, Fayetteville, AR;Computer Science, University of Memphis, Memphis, TN;Biological Engineering, University of Arkansas, Fayetteville, AR;Computer and Information Sciences, University of Delaware, Newark, DE;Chemistry and Biochemistry, University of Delaware, Newark, DE;Biological Engineering, University of Arkansas, Fayetteville, AR;Chemistry and Biochemistry, University of Delaware, Newark, DE

  • Venue:
  • DNA'04 Proceedings of the 10th international conference on DNA computing
  • Year:
  • 2004

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Abstract

Libraries of DNA oligonucleotides manufactured by an In Vitro selection protocol were characterized for their non-crosshybridizing properties. Cloning and sequencing after several iterations of the protocol showed that the sequences, in general, became more non-crosshybridizing. Gel electrophoresis of protocol product, also, indicated non-crosshybridization, and showed evolution in the population of molecules under the non-crosshybridization selection pressure. Melting curves of protocol product also indicated non-crosshybridization when compared to control samples. Thus, it appears that the protocol does select populations of non-crosshybridizing sequences.