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In this paper, we describe a general methodology for reconstruction and animation of volumetric deformable objects. In the scope of a medical application, we want to simulate the motion and the form alteration of the internal anatomical organs. Multi-layer particle systems are well adapted to represent the geometry. However, several problems occur related to the structure of our model and the nature of internal forces. For this, we have introduced the necessary tools to overcome these problems and to handle the dynamic and deformable behavior of cancerous tissues and organs. Our particle system seems to be suitable to be integrated in radiation dose evaluation software, developed within the European BIOMED2-ARROW project by North Western Medical Physics team, in Manchester.