Volume microscopy of biological specimens based on non-confocal imaging techniques

Authors:
Stephen L. Senft;Vincent J. Argiro;William L. VanZandt
Affiliations:
Washington University, Saint Louis, Missouri;Maharishi International University, Fairfield, Iowa;Maharishi International University, Fairfield, Iowa
Venue:
VIS '90 Proceedings of the 1st conference on Visualization '90
Year:
1990

Citing 3
Cited 1

Display of Surfaces from Volume Data

IEEE Computer Graphics and Applications
Interactive volume rendering

VVS '89 Proceedings of the 1989 Chapel Hill workshop on Volume visualization
Volume rendering

SIGGRAPH '88 Proceedings of the 15th annual conference on Computer graphics and interactive techniques

Towards a comprehensive volume visualization system

VIS '92 Proceedings of the 3rd conference on Visualization '92

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Abstract

The newly available confocal light microscopes produce series of aligned images that implicitly constitute volumes. This would directly provide scientists with the capability for volumetric study of the biological world at high spatial resolution - except that methods to effectively visualize volume data have been insufficiently rapid and flexible. We have developed software research tools to fill that need. In the process, we have found that excellent reconstructions could be made from serial sections acquired using a charge-coupled device and a conventional light microscope (modified after Inoué [1]). Brightfield images of high-contrast subjects (such as golgi-impregnated or horseradish peroxidase labeled cells) were reconstructed crisply, as were some fluorescently labeled cells. Volume microscopy now should be within reach of, and of use to, a wider range of scientists.