State Space Realization of a Three-dimensional Image Set with Application to Noise Reduction of Fluorescent Microscopy Images of Cells

  • Authors:
  • Raimund J. Ober;Xuming Lai;Zhiping Lin;E. Sally Ward

  • Affiliations:
  • Aff1 Aff2;Aff1 Aff2;School of Electrical and Electronic Engineering, Nanyang Technological University, Singapore, Republic of Singapore 639798;Aff1 Aff2

  • Venue:
  • Multidimensional Systems and Signal Processing
  • Year:
  • 2005

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Abstract

A method is presented to calculate state space realizations of a three-dimensional image set. It is based on interpreting the image set as the impulse response of a 3D separable system. As an application it is shown how this method, combined with approximation steps, including balanced model reduction, can be used to suppress noise in three-dimensional image sets. The approach was motivated by a practical problem in the analysis of three-dimensional fluorescent microscopy image data of fluorescently labelled cells. The method is illustrated by an analysis of simulated data and experimental data. The proposed approach can also be applied to a two-dimensional image in a straightforward way.