Flexibility description of the MET protein stalk based on the use of non-uniform B-splines

  • Authors:
  • Magnus Gedda;Stina Svensson

  • Affiliations:
  • Uppsala University, Swedish University of Agricultural Sciences, Centre for Image Analysis, Uppsala, Sweden;Uppsala University/Swedish University of Agricultural Sciences, Uppsala, Sweden

  • Venue:
  • CAIP'07 Proceedings of the 12th international conference on Computer analysis of images and patterns
  • Year:
  • 2007

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Abstract

The MET protein controls growth, invasion, and metastasis in cancer cells and is thereby of interest to study, for example from a structural point of view. For individual particle imaging by Cryo-Electron Tomography of the MET protein, or other proteins, dedicated image analysis methods are required to extract information in a robust way as the images have low contrast and resolution (with respect to the size of the imaged structure). We present a method to identify the two parts of the MET protein, β-propeller and stalk, using a fuzzy framework. Furthermore, we describe how a representation of the MET stalk, denoted stalk curve, can be identified based on the use of non-uniform B-splines. The stalk curve is used to extract relevant geometrical information about the stalk, e.g., to facilitate curvature and length measurements.