Cascading Whiplash PCR with a Nicking Enzyme

  • Authors:
  • Daisuke Matsuda;Masayuki Yamamura

  • Affiliations:
  • -;-

  • Venue:
  • DNA8 Revised Papers from the 8th International Workshop on DNA Based Computers: DNA Computing
  • Year:
  • 2002

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Abstract

Whiplash PCR has been proposed as a unique mechanism realizing autonomous inference machines and used in various applications of DNA coumputing. However, it is not easy to increase step sizes within a single molecule because of back annealing. This paper proposes a sheme to cascade results of WPCR from molecules to molecules by using a nicking enzyme. We also show preliminary experiments to produce output fragments continuously from WPCR.